New generation of best-quality sperm selection
Prague Fertility Centre introduce a new generation of Magnetic-Activated Cell Sorting (MACS) system – an efficient method to select functional sperm and improve pregnancy rates.
Genetic integrity of the spermatozoon is essential for normal embryo development. A high level of DNA fragmentation in sperm cells can negatively affect embryo cleavage and subsequent development leading to blastocyst arrest or early miscarriage. DNA damage represented by fragmentation and subsequent sperm apoptosis may be a cause of male infertility that standard methods – sperm concentration, morphology assessment and motility analysis cannot detect.
A major causative factor for sperm DNA damage is oxidative stress generally increasing with the age and/or inflammatory infections, cigarette smoking, drug use, exposure to environmental pollutants and elevated testicular temperature.
Therefore, MACS® ART Annexin V System was designed to selectively remove these defective although morphologically indistinguishable cells from sperm preparations. The procedure begins with magnetic labeling of unwanted cells and then, they are passed through a separation column where they are selectively retained. Intact living spermatozoa without DNA fragmentation pass through the column and are collected for later use (ICSI or cryopreservation).
Gain of the sperm ready for fertilization represents the key advantage over traditional DNA fragmentation tests (eg. SCSA/SDIA, see Tab.) in which patient obtain only impractical information on percentage of damaged cells. Similarly, methods for morphological assessing, like IMSI, cannot provide sufficient picture about the state of the DNA in the sperm nucleus.
Tab.:Table of selected techniques for the assessment of sperm quality
|MACS||Magnetic-Activated Cell Sorting||Magnetic nanoparticle-based selection of live sperm with integral (not fragmented) DNA|
|PICSI||Petri-dish Intracytoplasmic Sperm Injection||Binding to hyaluronic acid as an indication of sperm maturity|
|IMSI||Intracytoplasmic Morphologically Selected Sperm Injection||High magnification, motile sperm organellar morphology examination|
|SCSA/SDIA||Sperm Chromatin Structure Assay/Sperm DNA Integrity Assay||Fluorescently-based determining the percentage of sperm with fragmented DNA and the degree of DNA damage|