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Selection of viable spermatozoa by Laser-Assisted Immotile Sperm Selection (LAISS)

Method, which uses a laser to identify viable sperm cell for subsequent ICSI, recommended in MESA/TESE IVF cycles and any other cases of immotile spermatozoa.

Sperm viability is a prerequisite for a successful ICSI treatment, because the injection of a nonviable spermatozoon into an oocyte generally results in fertilization failure. To identify viable sperm in patients presenting with complete sperm immotility is a challenging but very important step. Using LAISS a single laser shot is applied close to the tip of the sperm tail using a non-contact 1.48 µm diode laser system. In a few seconds, two possible reactions can be observed:

  1. either the tail of the spermatozoa starts curling after the laser shot, or
  2. the spermatozoa show no reaction at all.

Spermatozoa showing a curling reaction are considered to be viable and can be used for ICSI treatment immediately. In contrast to another methods e.g. hypo-osmotic swelling test, the laser selection can be performed directly in IVF medium with no additional micromanipulation.

LAISS is regarded as a safe procedure since a shot applied to the far end of the flagellum does not affect the sperm head containing the genetic material. Moreover, this novel technique used in a specific group of patients increase the chance to select viable spermatozoa capable of fertilization.

Read more about LAISS on Fertilitypedia

Prague Fertility Centre recommendation…

…generally, we recommend laser-assisted immotile sperm selection in patients undergoing surgical sperm retrieval (MESA, TESE), or in patients diagnosed with sperm immotility (asthenozoospermia, oligoasthenote­ratozoospermi­a, necrozoospermia).

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