Treatment methods

Our goal is not only a positive pregnancy test. We want to make sure that the entire treatment process leads to a healthy pregnancy and baby.

IVF (In Vitro Fertilization) is a process of fertilization outside the body. The goal is to reach fertilization by placing sperm and oocytes together and letting them interact “in vitro”. The process is technically similar to regular fertilization, except it occurs in a laboratory environment.

However, this method of spontaneous fertilization that has been commonly used in IVF treatments can bring some challenges such as lower fertility rate or a penetration of more than one sperm into the egg. For these reasons, specific laboratory methods of fertilization are implemented in IVF treatments in order to eliminate the challenges we mentioned.

.01

IVF with own oocytes.

Every couple we treat is unique. Correct evaluation and diagnosis by our doctors ensures that the treatment methods they recommend will specifically suit you. Carefully chosen methods pave the way to our goal: your healthy and successful pregnancy.

.02

IVF with donation.

IVF with donated egg cells is proven to be a very successful fertility treatment. Oocytes from an anonymous and healthy donor get fertilized using your partner’s or donor’s sperm. The embryos, after the cultivation period, are transferred into the recipient’s womb. The remaining embryos are frozen for future transfers.

See donation programmes
Icon IVF

Our
methods

.01

Sperm selection
and fertilization

ICSI - Intracytoplasmic Sperm Injection +

Intracytoplasmic Sperm Injection

ICSI is a procedure used in IVF treatment that dramatically increases the chance of egg fertilization. It has become the most common method of fertilization and it is a standard fertilization method in our clinic.

We prefer to do it even when the sperm analysis of the male partner is normal. ICSI is performed by injecting a single sperm cell into an oocyte and it has proven revolutionary in solving the problems of male infertility. Dr. Daniel Hlinka, one of Prague Fertility Centre founders, was the first to perform ICSI method that resulted in the birth of a healthy baby in 1994.

PICSI - Preselected Intracytoplasmic Sperm Injection +

Preselected Intracytoplasmic Sperm Injection

PICSI is a method that is very similar to ICSI. The major difference is the way in which sperm is selected for fertilization.

With ICSI, the sperm gets selected on the basis of its movement and appearance, but with PICSI, we select only mature sperm that is judged by its ability to bind with hyaluronan microdots. PICSI provides us with the mature and morphologically correct sperm for injection into an oocyte.

LAISS - Laser-Assisted Immotile Sperm Selection +

Laser-Assisted Immotile Sperm Selection

LAISS is a method that has proven beneficial in identifying immotile spermatozoa if they are alive and viable for ICSI method fertilization.

Sperm viability is a prerequisite for successful ICSI treatment, because the injection of a nonviable spermatozoon into an oocyte generally results in fertilization failure. Method is recommended in MESA/TESE IVF cycles and any other cases of immotile spermatozoa.

MACS - Magnetic-Activated Cell Sorting +

Magnetic-Activated Cell Sorting

MACS is a method of sperm-selection using magnetic field technology designed to help selectively remove DNA defective sperm.

The procedure begins with magnetic labelling of unwanted cells and then they are passed through a separation column where they are selectively retained. Living spermatozoa without DNA defection pass through to be used later for ICSI fertility method or cryopreservation.

.02

Embryo selection
for transfer

EmbryoScope® +

Selecting good-quality embryos for the transfer is one of the key processes in IVF treatment.

Making a good choice of embryos can save the patients both time and money and ensures an easier route to the desired pregnancy. That is why we use EmbryoScope for embryo monitoring. Benefits of EmbryoScope:

  • noninvasive, real-time monitoring and evaluation of embryos
  • ability to reveal defects in cellular division of embryos
  • ability to discover poor-quality embryos and discard them before the transfer

Thanks to EmbryoScope, it is possible to monitor and fully control embryo development while selecting the best ones for transfer or freezing.

CATI - Cognitive Automation of Time-lapse Images +

Cognitive Automation of Time-lapse Images

Information provided by observation of early stage embryo development is the key to creating a fertility treatment plan. For that purpose we designed CATI (Cognitive Automation of Time-lapse Images) - the world's first automated system for evaluating and selecting embryos with the help of artificial intelligence.

It allows us to observe the vitality and genetic constitution of an embryo and diagnose the origin of any errors in the cell division of embryos. Thanks to CATI we have significantly increased the effectiveness of our PGD/PGS methods and reduced the risk of miscarriage or the risk of having an unhealthy baby. In this way, we not only save money of our patients, but more importantly we help them avoid a stressful experience.

Prolonged Cultivation - Prolonged Embryo Cultivation Period +

Prolonged Embryo Cultivation Period

Cultivation period of an embryo in lab environment is usually around 3 days which is the time period from the fertilization and the transfer.

In certain cases, embryologists recommend prolonged cultivation in order to select the best embryos for the transfer, especially in cases when the doctor recommends single embryo transfer and there are many embryos to choose from. Prolonged cultivation period, which lasts 5 days, allows us to discard, with more ease and accuracy, the embryos that develop in an unsatisfactory manner and choose the good-quality ones for the transfer and freezing.

.03

Assistance for
embryo implantation

LAZT - Laser-Assisted Zona Thinning +

Laser-Assisted Zona Thinning

An embryo is surrounded by a layer called the zona pellucida and when the embryo starts hatching this layer starts to get thinner and eventually decomposes. When performing laser-assisted zona thinning, we make sure that the embryo spends less energy when hatching by getting out of its shell with more ease and eventually implanting itself.

The embryologists use laser technology in order to make the zona pellucida thinner. This method ensures embryo implant more easily which brings more chances for a successful pregnancy.

EmbryoGen® +

EmbryoGen® is a new advanced embryo culture medium which includes a natural communication substance used by the mother during pregnancy.

The embryo is cultured in EmbryoGen® during the IVF treatment process and is finally returned to the woman’s uterus along with a small amount of EmbryoGen®. This way the embryo is better prepared for dialogue when returned to the uterus.

EmbryoGlue® +

EmbryoGlue is a medium used exclusively during transfer and its use has been shown to increase the chances for successful implantation of an embryo.

EmbryoGlue contains hyaluronan and recombinant human albumin and it mimics the conditions of the uterus. The medium makes communication between embryos and the endometrium easier and helps embryos implant after transfer.

.04

Endometrial receptivity
and implantation window

ASET - Asynchronous Embryo Transfer +

Asynchronous Embryo Transfer

ASET is recommended by our doctors when they suspect that the reason for the patient not getting pregnant, is due to the endometrial factor. It can happen that the endometrium is not receptive to the embryo as the window of the implantation of the endometrium can vary from cycle to cycle.

With this method, what we do is transfer different embryos of different age by postponing some of them before the transfer with vitrification. By transferring the embryos of different age, we help the endometrium be more receptive in the hope of successful implantation.

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