ICSI Fertility Treatment In Prague, Czech Republic - PFC

Intracytoplasmic sperm injection (ICSI)

Intracytoplasmic sperm injection or ICSI is one of the most effective laboratory techniques of modern assisted reproduction. The best quality sperm, selected by advanced methods, are gently injected into the egg during ICSI. This can overcome a number of barriers and increase the chance of successful fertilisation.

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What is ICSI?

Injecting sperm directly into the egg can remove obstacles to pregnancy and increase the chances of fertilisation

We perform ICSI in our laboratory using a micromanipulator device. The micromanipulator transforms imperfect hand movement into extremely careful and precise movement. Using the device we can gently inject the selected sperm into the egg. The fertilised egg is then placed in an incubator, where it develops over the next few days in favourable conditions, at a temperature of 37°C, 6 % CO2 and 6 % O2.


Physiological intracytoplasmic sperm injection (PICSI) is an older method that involves placing sperm with hyaluronic acid, a natural compound found in the body. PICSI identifies sperm that can bind to HA and these sperm are selected for use in treatment. In PFC laboratories, we no longer use this method. Sperm suitable for fertilisation are selected using modern laboratory techniques, such as MICHSS or LAISS, and are subsequently used for fertilisation using the ICSI method. 

The micromanipulator is a highly complex device. Its operation, performed while simultaneously looking into a microscope, is a truly intricate technique that requires calmness and concentration. In PFC laboratories, highly experienced and skilled embryologists perform the ICSI micromanipulation technique with care, skill and unwavering diligence. Due to these innovative methods of sperm selection, we achieve a high success rate in egg fertilisation.

Main benefits of ICSI

  • ICSI increases the chances of fertilization by 60 – 80% and successful pregnancy by 30 – 50%.
ICSI detail mikroskop

ICSI close-up view I PFC

To learn more about our personal, bespoke treatment and options for combining IVF methods at PFC, please contact our IVF coordinator to arrange a personal consultation.