Intracytoplasmic sperm injection or ICSI is one of the most effective laboratory techniques of modern assisted reproduction. The best quality sperm, selected by advanced methods, are gently injected into the egg during ICSI. This can overcome a number of barriers and increase the chance of successful fertilisation.
Injecting sperm directly into the egg can remove obstacles to pregnancy and increase the chances of fertilisation
We perform ICSI in our laboratory using a micromanipulator device. The micromanipulator transforms imperfect hand movement into extremely careful and precise movement. Using the device we can gently inject the selected sperm into the egg. The fertilised egg is then placed in an incubator, where it develops over the next few days in favourable conditions, at a temperature of 37°C, 6 % CO2 and 6 % O2.
Physiological intracytoplasmic sperm injection (PICSI) is an older method that involves placing sperm with hyaluronic acid, a natural compound found in the body. PICSI identifies sperm that can bind to HA and these sperm are selected for use in treatment. In PFC laboratories, we no longer use this method. Sperm suitable for fertilisation are selected using modern laboratory techniques, such as MICHSS or LAISS, and are subsequently used for fertilisation using the ICSI method.
The micromanipulator is a highly complex device. Its operation, performed while simultaneously looking into a microscope, is a truly intricate technique that requires calmness and concentration. In PFC laboratories, highly experienced and skilled embryologists perform the ICSI micromanipulation technique with care, skill and unwavering diligence. Due to these innovative methods of sperm selection, we achieve a high success rate in egg fertilisation.
ICSI close-up view I PFC
To learn more about our personal, bespoke treatment and options for combining IVF methods at PFC, please contact our IVF coordinator to arrange a personal consultation.
Preimplantation genetic screening and diagnosis — prevention of nesting failure and transmission of genetic defects.
Modern microsurgical sperm collection methods from the testicles and epididymis in the case of low / zero sperm concentration.
Selection of the best quality sperm based on concentration and motility using a microfluidic chip.
An innovative polarising microscopy method for monitoring egg maturity to determine the correct timing for fertilisation.
Monitored embryo development over 48 hours to select the embryo with the highest potential for implantation.
A real-time embryo development tracking system to select the embryos with the highest potential for nesting.
A special solution for protecting the embryo and facilitating its connection with the uterine lining.
Laser-assisted hatching is a non-invasive method for facilitating an embryo to leave the protective shell and promote nesting.
Asynchronous embryo transfer is a method in which we transfer 2 embryos of different ages to increase chances of succesful implantation.
ERA test, beReady — advanced analytical methods of the genetic profile of the uterine lining to determine the timing of implantation.
Preservation of eggs and embryos for potential future use by fast, gentle and efficient freezing.